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. 2019 Nov 19;9:17104. doi: 10.1038/s41598-019-53176-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mAb-513-induced DDR1 redistribution and phosphorylation. (A) COS-7 cells transiently expressing DDR1 were either incubated with mAb-513 IgM for the indicated times at 37 °C or left unstimulated, then incubated on ice with anti-DDR1 mAb 7A9, before fixation, permeabilisation and immunostaining for phospho-tyrosine 513 (pY-DDR1). Cells were imaged using a widefield microscope. White boxes in DDR1 images indicate corresponding areas shown at higher magnification in images to the right. Scale bars, 30 μm or 10 μm (magnification). (B) Cells were treated and imaged as above. Mean pY-DDR1 signal for each cell was calculated and averaged for the stimulation time. Values were normalised so that the mean value for 0 and 60 minutes was 0 and 100 A.U respectively. Error bars are SEM. N = 15–23.