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. 2019 Nov 19;9:17094. doi: 10.1038/s41598-019-53519-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The C-terminal PDZ-binding motif of Slitrk2 is required for Slitrk2 binding to PDZ domains of PSD-95 or the PDZ domain of Shank3a. (A) Schematic diagrams of a series of PSD-95 deletion constructs. Abbreviation: PDZ, PSD-95/Dlg-A/ZO-1; SH3, src homology 3; and GK, guanylate kinase. (B) Coimmunoprecipitation experiment demonstrating that PDZ1 + 2 domains of PSD-95 are required for interaction with Slitrk2. HEK293T cells were transfected with HA-tagged Slitrk2 alone or together with the indicated PSD-95 deletion constructs, after which coimmunoprecipitation of Slitrk2 with PSD-95 was assayed. Input, 5%. (C) Schematic diagrams of Shank3a, Shank3b, Shank3c, and Shank3e expression vectors. Abbreviations: ANK, ankyrin repeat; PDZ, PSD-95/Dlg-A/ZO-1; SH3, Src homology 3; and SAM, sterile alpha motif. (D) HEK293T cells were transfected with HA-tagged Slitrk2 alone or together with Shank3 variants (C), after which coimmunoprecipitation of Slitrk2 with Shank3 was assayed. Input, 5%. (E) Schematic diagrams of a series of Shank3a deletion constructs. Abbreviations: ANK, ankyrin repeat; PDZ, PSD-95/Dlg-A/ZO-1; SH3, Src homology 3; and SAM, sterile alpha motif. (F) Coimmunoprecipitation experiment demonstrating that the PDZ domain of Shank3a is sufficient for interaction with Slitrk2. HEK293T cells were transfected with HA-tagged Slitrk2 alone or together with Shank3a deletion constructs, after which coimmunoprecipitation of Slitrk2 with Shank3a was assayed. Input, 5%.