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. 2019 Nov 19;9:17077. doi: 10.1038/s41598-019-53710-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representations of the melon CmPDS target gene (MELO3C017772.2 in ICUGI data base), location of the gRNA1 and gRNA2, and the CRISPR/Cas9 vector. (A) Schematic representation of melon CmPDS gene with two target sites indicated as bold and PAM sequences as underlined. (B) Representation of CmPDS target sequences. Exons are shown as capital letters, introns as lowercase, gRNA1 and gRNA2 as bold, PAM sequences as underlined, and primers used for gene sequencing as italics. (C) Schematic representation of the CRISPR/Cas9 binary vector used for melon transformation. Arabidopsis thaliana promoters and terminators drive expression of gRNA1(AtU6–26p and AtU6–26t) and gRNA2 (AtU6–29p and AtU6–29t). The Cauliflower mosaic virus promoter (CaMV 35S) drives the expression of the Cas9 gene.