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. 2019 Nov 19;9:17077. doi: 10.1038/s41598-019-53710-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Targeted mutations detected in melon plants and protoplasts induced by CRISPR/Cas9 in each gRNA. Target sequences of CmPDS are bolded and the protospacer adjacent motif (PAM) in italics. Aligned sequence data is shown for four plants (number) and protoplasts (P). Number of colonies sequenced are indicated in the left between parentheses. Substitutions are highlighted in yellow; insertions are highlighted in blue, whereas a · is used in sequences with no insertion to maintain the reading frame; and, deletions are highlighted in red.