Figure 5.

DITH and TEM analysis of the effect of PF74 on the stability of HIV-1 CANC. (a) An enlargement of a graph (Fig. S3) showing fluorescence emission curves demonstrating the kinetics of tqON release from preassembled wt CANC particles incubated in assembly buffer (black curve) and disassembly buffer (red curve) in the absence of PF74 or in the disassembly buffer at increasing concentration of PF74: yellow (4 µM), pink (6 µM), violet (8 µM), salmon (10 µM), brown (16 µM), green (18 µM) and blue (24 µM). The stabilization effect of PF74 was calculated as the difference between the relative fluorescence of tqON at 90 min in the disassembly and assembly reactions according the calculation: relative percent of stabilization = 100*Δ2/Δ1. (b) DITH quantification of the relative stability of preassembled wt particles incubated in the disassembly buffer in the presence of increasing amount of PF74, measured and calculated as described in (a). Relative stability of wild type in disassembly buffer is considered as 0%. Relative percent of E45A CANC mutant stability in the disassembly buffer, measured and calculated as it is shown in 4 g, was added. (b,c) Following DITH, an untreated sample (b) and the sample containing 18 µM PF74 (c) were negatively stained and analyzed by TEM.