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. 2019 Nov 19;9:17076. doi: 10.1038/s41598-019-53483-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of IP6 on the relative stability of CANC HIV-1 particles compared to effect of E45A mutant and PF74 determined by DITH. Tubular particles were assembled from HIV-1 CANC in the presence of tqON and indicated concentrations of IP6 in assembly buffer. Samples were incubated for 3 h and then diluted into assembly or disassembly buffers and incubated overnight. (a) Fluorescence emission curves demonstrating the kinetics of tqON release from preassembled wt CANC particles incubated in assembly buffer (black curve) and disassembly buffer (red curve). The relative stabilization for various IP6 concentrations (from 0.7–3.0 µM) was calculated as the difference between the relative fluorescence of tqON at 90 min in disassembly and assembly reactions according the calculation: relative percent of stabilization = 100 * Δ2/Δ1. (b) DITH quantification of the relative percent of stability of preassembled wt particles incubated in disassembly buffer in the presence of increasing amount of IP6, measured and calculated as described in (a). Relative percent of E45A CANC mutant stability and wt CANC in the presence of 10 µM PF74 in the disassembly buffer is also shown.