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. 2019 Nov 13;10:2638. doi: 10.3389/fimmu.2019.02638

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Copyright © 2019 Fu, Jiang, Hao, Liu, Ding, Zhang, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M1 and M2 mobilization in the lung of signal transducer and activator of transcription 6 (STAT6)-deficient mice. (A,B) Fluorescent images of CD68 (green) and CD163 (red) in lung carcinoma and para-carcinoma tissues of WT and STAT6^−/− tumor-bearing mice. (C) Flow cytometry analysis of M1 and M2 cells. (D–G). M1 and M2 cells in WT and STAT6^−/− tumor-bearing mice. (D) Percentage of M1 cells (CD11b⁺ F4/80^low Ly6C^highCD206⁻) in the blood. (E) Percentage of M2 cells in the blood (CD11b⁺ F4/80^highLy6C⁻CD206⁺). (F) Percentage of M1 cells in the lung. (G) Percentage of M2 cells in the lung. (H) Relative mRNA expression of iNOS in CD11b⁺cells isolated from the lung. (I) Relative mRNA expression of Arg1 in CD11b⁺ cells directly obtained from mice. Total number of gated cells was 10 × 10E4. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.