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. 2019 Jul 12;101(4):704–718. doi: 10.1093/biolre/ioz113

Figure 2.

Figure 2.

Range of TIR1-myc cRNA concentrations allows for depletion of exogenously expressed AIDm-EGFP. Prophase I oocytes were microinjected with 0.4 μg/μl AIDm-EGFP and 2.2, 1.1, or 0.5 μg/μl TIR1 cRNA 6 h prior to 500 μM IAA treatment. (A) Live-cell EGFP fluorescence (gray) in prophase I oocytes 20 min prior to (−20 min) and 180 min after IAA addition. (B) Graphical representation of EGFP fluorescence over time. 500 μM IAA was added at 0 min (arrow). EGFP fluorescence normalized to expression at −180 min. (C) Graphical comparison of EGFP fluorescence before (−20 min) and after (180 min) IAA addition. EGFP fluorescence was normalized to expression at −20 min (ANOVA with Tukey's post hoc). n = number of oocytes analyzed (15–29 over 2–3 technical replicates), P ≤ 0.0391. Line graph shows mean and SEM of EGFP fluorescence every 20 min. Bar graph shows mean, SEM, and individual oocyte scatter plot.