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. 2019 Jul 12;101(4):704–718. doi: 10.1093/biolre/ioz113

Figure 6.

Figure 6.

NAA induces auxin-inducible degradation to the same extent as IAA. Oocytes were injected with 0.4 μg/μl AIDm-EGFP cRNA and 2.0 μg/μl TIR1-myc cRNA, and allowed to express cRNA for 6 h prior to 500 μM IAA or NAA treatment. (A) Live-cell EGFP fluorescence (gray) in prophase I oocytes 20 min prior to (−20 min) and 180 min post IAA or NAA addition. (B) Graphical representation of EGFP fluorescence over time in vehicle, IAA, or NAA-exposed oocytes. Auxin was added at a concentration of 500 μM at t = 0 min (arrow). EGFP fluorescence normalized to expression at −180 min. (C) Graphical comparison of EGFP 180 min after auxin addition. EGFP fluorescence normalized to expression at −20 min (Kruskal–Wallis test with Dunn's post hoc). n = number of oocytes analyzed (39–46 over four technical replicates), P < 0.0001. (D, E) Graphical representation of percentage of oocytes at MII following 16 h of IVM and their time spent in meiosis I (D: Fisher's exact test; E: Mann-Whitney test). Bar graphs show mean, SEM, and individual oocyte scatter plot (C) or percentage of oocytes that emitted a first polar body (D). Line graph shows mean and SEM of EGFP fluorescence every 20 min. Scatter plot shows mean and individual oocyte meiosis I timing.