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. 2019 Jul 12;101(4):704–718. doi: 10.1093/biolre/ioz113

Figure 7.

Figure 7.

Increased auxin concentration has no impact on loss of exogenously expressed AIDm-EGFP. (A, B) Oocytes were injected with 0.4 μg/μl AIDm-EGFP cRNA and 2.0 μg/μl TIR1-myc cRNA, and allowed to express cRNA for 3 h prior to start of imaging. (A) Graphical representation of EGFP fluorescence over time in prophase I oocytes treated with vehicle, 500 μM, or 1000 μM NAA. NAA was added at 0 min (arrow). EGFP fluorescence normalized to expression at −180 min. (B) Graphical comparison of EGFP fluorescence before (−20 min) and after (180 min) NAA addition. EGFP fluorescence was normalized to expression at −20 min (Kruskal–Wallis test with Dunn's post hoc). n = number of oocytes analyzed (23–26 over three technical replicates), P ≤ 0.0001. (C, D) Oocytes were injected with 0.4 μg/μl AIDm-EGFP cRNA and 2.0 μg/μl TIR1-myc cRNA, and allowed to express protein from the injected cRNA for 5 h prior to live-cell imaging. (C) Graphical representation of EGFP fluorescence over time in prophase I oocytes treated with vehicle, 500 μM, 1000 μM, or 2000 μM IAA. IAA was added at 0 min (arrow). EGFP fluorescence was normalized to expression at −20 min. (D) Graphical comparison of EGFP fluorescence before (−20 min) and after (180 min) IAA addition. EGFP fluorescence was normalized to expression at −20 min (Kruskal–Wallis test with Dunn's post hoc). n = number of oocytes analyzed (14–28 over two technical replicates), P ≤ 0.0001. Line graphs show mean and SEM of EGFP fluorescence every 20 min (B) or 180 min (D). Bar graph shows mean, SEM, and individual oocyte scatter plot in auxin-exposed and control oocytes.