Table 1.
Sequences and concentration of HPV16, HPV18 and β-globin primers and probes used in real-time PCR
Oligonucleotide | Sequence | Concentration (µM) | Product size (bp) |
---|---|---|---|
HPV16 E7 F | 5′-AGGAGGATGAAATAGATGG-3′ | 0.4 | |
HPV16 E7 R | 5′-CTTCCAAAGTACGAATGTC-3 | 0.4 | 141 |
HPV16 E7 probe | FAM-CACAACCGAAGCGTAGAGTCA-BHQ | 0.16 | |
HPV18 E7 F | 5′-CGATGAAATAGATGGAGTTAA-3′ | 0.4 | |
HPV18 E7 R | 5′-CACACTTACAACACATACA-3′ | 0.4 | 92 |
HPV18 E7 probe | JOE-ACATTTACCAGCCCGACGAG-BHQ | 0.16 | |
β-globin F | 5′-TGTGTTCACTAGCAACCTCAA-3′ | 0.4 | |
β-globin R | 5′-CTCACCACCAACTTCATCCA-3′ | 0.4 | 111 |
β-globin probe | FAM-CCTGAGGAGAAGTCTGCCGTTACTGCC-BHQ | 0.16 |