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. 2019 Aug 20;35(6):1024–1034. doi: 10.1007/s12264-019-00421-5

Fig. 1.

Fig. 1

Effects of Gedunin on mHTT and Hsp90 chaperones in vitro. A, B Immunoblots (A) and statistics (B) showing a dose-dependent decrease of mHTT expression after treatment with Gedunin (5 μmol/L, 10 μmol/L, 15 μmol/L, and 20 μmol/L) in Neuro-2a cells transfected with EGFP-HD-Q74. The levels of Hsp70, p23 and Hsp90 were slightly decreased but the difference was not statistically significant (n = 3; mean ± SEM; P < 0.05, one-way ANOVA). C Representative images showing the level of EGFP-Q74 in transfected Neuro-2a cells after treatment with 20 μmol/L Gedunin for 24 h. Scale bar, 50 μm. D Immunoblots and results for MW1 antibody showing a time-dependent decrease of mHTT in EGFP-HD-Q74 transfected Neuro-2a cells after treatment with 20 μmol/L Gedunin (n = 3; mean ± SEM; P < 0.05, one-way ANOVA). E Treatment with Gedunin did not affect mHTT RNA in EGFP-Q74-transfected Neuro-2a cells.