Knocking down MAN1 lengthens the period of the locomotor rhythm. A Double-plotted representative actograms of the indicated genotypes. Flies were monitored in LD for 4 days and then DD for 7 days. Gray shades indicate dark phases. B–E The period (B, C) and power (D, E) of the DD locomotor rhythm of flies with MAN1 knocked down and controls. Digits on the bars are the number of flies tested. Percentage of rhythmicity is indicated above the bars (P < 0.001, one-way ANOVA; ***/###P < 0.001, Tukey’s multiple comparison test). F Plots of relative mRNA abundance of MAN1 in whole head extracts of flies with MAN1 knocked down and controls determined by qRT-PCR (n = 3; */#P < 0.05, **/##P < 0.01, ***P < 0.001 * vs GAL4 controls, #vs UAS controls, Student’s t test). Error bars represent the standard error of the mean (SEM). G4, GAL4; U, UAS.