Fig. 2.

Knocking down MAN1 in adults lengthens the period of the locomotor rhythm. A, B Double-plotted representative actograms of the indicated genotypes. Flies treated with vehicle control (A) or RU486 (B) to activate the pdfG4-geneswitch (pdf-GS) driver. C, D The period and power of DD locomotor rhythms of flies with MAN1 knocked down and controls. Flies treated with vehicle control (C) or RU486 (D). Flies are monitored in LD for 4 days and then DD for 7 days. Gray shades indicate dark phases. Error bars represent SEM. Digits on the bars are the number of flies tested. Percentage of rhythmicity is indicated above the bars (P < 0.001, one-way ANOVA; ***^/###P < 0.001, * vs GAL4 controls; # vs UAS controls, Tukey’s multiple comparison test). G4, GAL4; U, UAS.