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Knocking down MAN1 reduces the pre-mRNA level of per. A Plots of relative pre-mRNA abundance of per determined by qRT-PCR in whole head extracts of MAN1RNAi (timG4/MAN1RNAi;Udcr2/+) and control (timG4/+;Udcr2/+) flies during DD1 (n = 4; *P < 0.05, Student’s t test). B Plots of relative mRNA abundance of MAN1 in whole head extracts of w1118 flies in DD1 determined by qRT-PCR (n = 3; P > 0.05, one-way ANOVA).
