Tdc2 motor neurons mediated light-induced deceleration. A Inhibiting Tdc2 motor neurons abolished larval preference for darkness (**P < 0.01, ***P < 0.001; n = 22, 23, 17, 19, 23, and 23 from left to right). B Acute inhibition of Tdc2 neurons abolished the preference for darkness (Heat shock, with restrictive temperature; No heat shock, with permissive temperature; ***P < 0.001, n.s., not significant; n = 29, 19, 47, 15, 19, and 28 from left to right). C, D Effect of inhibiting Tdc2 neurons on larval head casting and deceleration in response to light-spot entry. C The degree of head casting was decreased. D The minimum speed after deceleration did not differ from controls (**P < 0.01, n.s., not significant; n = 81, 52, and 92 in (C) and 82, 53, and 96 in (D) for Tdc2-Gal4, UAS-TNTG/+ and Tdc2 > TNTG, respectively). One-way ANOVA followed by post hoc Tukey’s multiple comparison test was used for A and B. Kruskal-Wallis test followed by post hoc Dunn’s multiple comparison test was used for C and D.