Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 19;9:17033. doi: 10.1038/s41598-019-53370-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(a) RT-qPCR analysis of CBX2.1 downstream genes identified by DamID. Relative expression levels (2^−ΔΔCt) of the genes were determined after normalization to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Following CBX2.1 overexpression (Ov Exp. CBX2.1), ESR1, NRG1, BMP2, PTGER2, and FZD7 were found downregulated by CBX2.1 compared to the control set at 1. After silencing the CBX2.1 (si CBX2.1), genes were significantly upregulated except for ESR1 which showed an effect comparable to scrambled sample. All graphs are the average of three independent experiments, error bars represent the standard deviation (SD) from the mean (SEM) and values are expressed as relative to control =1; ***P < 0.001; **P < 0.01 and *P < 0.05. non-significant differences are not indicated. (b) Relative expression levels (2^−ΔΔCt) of CBX2.1 related genes. POF1B, DKK1 and SOX9 were upregulated after CBX2.1 forced expression (Ov. Exp CBX2.1). Whereas, when CBX2.1 was silenced (si CBX2.1), SOX9 and POF1B were significantly downregulated. DKK1 did not show any expression change towards the siCBX2.1. All graphs are the average of three independent experiments, error bars represent SD from the mean (SEM), and values are expressed as relative to control =1; ***P < 0.001; **P < 0.01 and *P < 0.05. non-significant differences are not indicated.