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. 2019 Nov 19;9:17016. doi: 10.1038/s41598-019-53171-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surface Plasmon Resonance study. Biacore analysis revealed that BP1253 has the ability to bind AMP, GMP and IMP with a dissociation constant of 5.7, 38.6 and 71.8 µM respectively. BP1253-His was immobilized via amine coupling procedure on Sensor chip CM5. Concentrations from 0.78 to 400 μM were used to measure the K_D. Identical results were obtained by three independent experiments.