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. 2019 Nov 19;9:17016. doi: 10.1038/s41598-019-53171-9

Figure 8.

Figure 8

Deletion of BpLOG confers greater resistance to hydrogen peroxide. Wild-type Bp (black columns) and Δ1253 mutant Bp (grey columns), grown for 24 hours in Steiner-Scholte medium, were treated for 30 minutes with: (A) 100 mM or 300 mM H2O2, or left untreated; (B) 10 µM 6-O-methylguanine alone or in combination with 100 mM H2O2, or left untreated. At the end of the treatment, bacteria were diluted 1:109, plated on Bordet-Gengou agar plates and incubated at 37 °C for three days when the number of colonies was counted (CFU). Experiments were repeated three times. Mean CFU ± SD of Δ1253 and wild-type Bp are reported. *P < 0.05; ***P < 0.001, as measured by two-tailed Student’s t-test; ns, not significantly different.