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. 2019 Jul 18;101(4):782–790. doi: 10.1093/biolre/ioz126

Figure 6.

Figure 6

Nile red staining of lipid droplets in D3.5 control and Mcoln1−/− ovaries. (A) Control ovary. (B) A Mcoln1−/− ovary with mild lipid accumulation. (C) A Mcoln1−/− ovary with severe lipid accumulation. (A1–C1) Representative images of luteal cells enlarged from (A) to (C), respectively; green: Nile red staining of lipid droplets; blue, DAPI staining of nuclei; red #: corpus luteum; white *: follicle; scale bar: 400 μm (A–C) or 12.5 μm (A1–C1). (D) Number of lipid droplets in a standard reference frame (1041×780 pixel image). (E) Size of lipid droplets (square pixels). (D and E) N = 5; * P < 0.05; error bar, standard deviation.