Figure 8.
Detection of StAR mRNA expression in D4.5 ovaries by in situ hybridization using a StAR antisense probe. A. StAR expression in an ovary of a 3 months old pseudo-pregnant control mouse. B. StAR expression in an ovary of a 3 months old pregnant Mcoln1−/− mouse. C. StAR expression in an ovary of a 5 months old non-pregnant Mcoln1−/− mouse. A1-C1: Enlarged from the boxed area in A–C, respectively; red #: corpus luteum; black *: follicle; scale bar: 200 μm (A–C) or 50 μm (A1–C1); no specific staining in a control section using a StAR sense probe (data not shown).