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Knockdown of Setd1a significantly increased the mRNA expression of Neurl4 and Usp39. A Setd1a mRNA levels by RT-qPCR showing that sh1# was the most effective (one-way ANOVA, ***P < 0.001). B Neurl4 mRNA levels by qPCR (unpaired t test, *P = 0.0415). C Usp39 mRNA levels by qPCR (unpaired t test, ****P < 0.0001).
