Fig. 3.

Effect of the dichloromethane fraction (DF) on the production of pro-inflammatory mediators by LPS-stimulated RAW 264.7 macrophages. (A): NO•, (B): PGE2, (C): IL-1 β, (D): IL-6, (E): TNF-α, (F): MCP-1. Cells were incubated for 24 hours after stimulation and treatment with DF. Then supernatants were collected and mediators were measured through ELISA kits, except the NO• which was measured with the Griess reaction, as described in Methods. Each value represents mean ± SEM (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 compared with LPS group (One way ANOVA).