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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Biomater Sci. 2019 Nov 19;7(12):5338–5349. doi: 10.1039/c9bm00348g

Figure 1. Schematic representation of the preparation of cell-laden affinity-bound laminin PEG-4MAL hydrogels (not to scale).

Figure 1.

PEG-4MAL macromer is firstly functionalized with a thiol-containing mono-PEGylated rhNtA (NtA) domain, which will then mediate the binding of laminin with high affinity. Functionalized PEG-4MAL is then reacted with a mixture of protease degradable (MMP2-sensitive peptide) and non-degradable (PEG-dithiol) cross-linkers to allow the formation, under physiological conditions, of a hydrogel network in the presence of cells.