Figure 2. Effect of NtA concentration on laminin incorporation A).
Density of NtA tethered to PEG-4MAL hydrogel as a function of input NtA concentration. Linear regression: y = 0.9954 x + 0.1115; R2 = 0.9999. Data represent mean ± standard deviation (SD) of three hydrogels prepared independently. B) Laminin incorporation by physical entrapment (Entrap) or by immobilization using an affinity binding ligand (Immob) into PEG hydrogels, determined by ELISA, after 1 and 7 days of incubation in PBS. Data represent mean ± SD of three hydrogels prepared independently. No significant differences were detected (two-way ANOVA).