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Native mass spectra of streptavidin with 40 mM of charge reducing agent added. All spectra were collected at 0 V in-source trapping collision voltage except (D), which was 100 V. Additives were (A) water, (B) imidazole, (C, D) TMAO, (E) 4(5)-methylimidazole, (F) 2-methylimidazole, (G) 1-methylimidazole, (H) 4(5)-hydroxy methylimidazole, (I) 4(5)-ethylimidazole, (J) 2-ethylimidazole, (K) 2-propylimidazole, and (L) 2-isopropylimidazole.
