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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Prog Biophys Mol Biol. 2019 May 20;154:71–79. doi: 10.1016/j.pbiomolbio.2019.04.008

Figure 2: Adult mouse cardiomyocytes can be cultured in 3D PEG hydrogels for extended times.

Figure 2:

A) Representative brightfield and live images of adult mouse cardiac myocytes (AMVMs) cultured in 3D PEG hydrogels for 0, 1, 2, 3, or 5 days. Cells were cultured with calcein (Live) and ethidium homodimer for 1 hour prior to imaging. Scale bar = 200um. B) Representative brightfield and live images of AMVMs cultured on glass coverslips for 0, 1, 2 days. Cells were cultured with calcein and ethidium homodimer for 30 minutes prior to imaging. Scale bar = 200 um. C) Quantification of AMVM viability in 3D culture and glass coverlsips over 0, 1, 2, 3, or 5 days. For 3D culture N=3 mice, for glass N=4 mice. Two-way ANOVA with Bonferroni post-hoc test applied; *p<0.5 **p < 0.01. Data reported as mean ± SEM.