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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Immunol. 2019 Oct 25;203(11):2807–2816. doi: 10.4049/jimmunol.1900760

Figure 3: CREMα does not trans-activate the DUSP4 promoter.

Figure 3:

A) The DUSP4 gene is located on chromosome 8 and contains 5 exons. As a result of alternative splicing, DUSP4A and DUSP4B consist of 4 exons. The highly conserved DUSP4 proximal promoter (red box) harbors one almost complete consensus CREM recruitment element (CRE). We generated three luciferase reporter constructs of different lengths, all including the CRE. B) All reported constructs exhibit increased luciferase activity when compared to empty plasmids. C) Overexpression of CREMα through co-transfection of CREMα expression plasmids does not affect promoter activity of any of the three constructs. D) Luciferase activity of DUSP4 promoter constructs was not altered in CREM-deficient (D) or CREMα overexpressing (E) cell lines as compared to controls. In figures B-E means and standard deviations are displayed.