Figure 7.

Macrophage migration is mediated by hResistin-BTK. (A) Immunofluorescence staining for total BTK and F4/80 in lungs of mice after 4 days of hypoxia. Digitally merged signals are shown in the left panel, and separate channels are displayed in the right panels. Photographs shown are representative of three independent experiments with four individual lung samples. Magnification: 400X. (B) The total number of F4/80+ pulmonary macrophages that expressed BTK per high-power field (hpf, 400X) in (A). Data are presented as means ± SEM (n = 4 animals). *p < 0.05. (C) In vitro wound healing assay. Representative microphotographs show motility of human macrophages in scratch wound-healing model. THP-1-differentiated macrophages were treated with medium (control) or hResistin (100 and 200 ng/ml) for 16 h. In parallel, 200 ng/ml hResistin-stimulated macrophages were pretreated with LFM-A13 for 30 min. (D) Quantitative assessment of data from (C). Data are presented as means ± SEM (n = 6) and are representative of two independent experiments. *p < 0.05, **p < 0.01.