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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Immunol. 2019 Oct 21;203(11):2817–2826. doi: 10.4049/jimmunol.1900404

Figure 1: ABCs are a predominantly quiescent population with an extensive replication history.

Figure 1:

(A, B) KREC analysis of sorted naïve B cells (CD19+CD11b−CD11c−FAS−), CD38loFAS+ GC B cells, and CD11b+CD11c+ ABCs from 12-month-old C57BL/6 female (A) and autoimmune WAS chimera (B) mice. (C) % BrdU positive B cells in naïve, GC, and ABC compartments in 18-month-old aged female (left) and WAS chimera (right) mice. (A-C) Each data point indicates an individual animal, pooled from two independent experiments. *, P<0.05; ****, P<0.0001; by one-way ANOVA, followed by Tukey’s multiple comparison test. (D) FACS plots showing gating of GC B cells and ABCs (left panels), and histograms of BrdU labeling in indicated subsets (right panels) in representative 18-month-old C57BL/6 female (upper panels) and WAS chimera (lower panels) mouse. Number indicates % within gate.