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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Immunol. 2019 Oct 21;203(11):2817–2826. doi: 10.4049/jimmunol.1900404

Figure 2: ABCs express a diverse, somatically-mutated BCR repertoire.

Figure 2:

(A) Heavy chain (left; μ FM and ABC, γ WAS GC) and light chain (right; κ and λ) variable gene family usage among single sorted 3-month-old WT FM B cells (CD19+CD21intCD24int), ABCs from 8-month-old WT female mice, ABCs from autoimmune WAS chimeras (CD19+CD11b+CD11c+), and GC B cells (CD19+PNA+FAS+) from WAS chimera mice. (B) Amino acid usage in heavy and light chain variable genes from sorted ABCs from aged female mice, compared with control FM B cells from 3-month-old WT animals. (C) Mutation count in heavy and κ light chain variable genes in sorted single B cells from indicated genotypes. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 for each experimental population compared with WT FM control mutational frequency, by Kruskal-Wallis one-way ANOVA test. (D) Nucleotide substitution patterns in mutated sequences from sorted aged WT and autoimmune WAS chimera ABCs. Numbers indicate percentage of each specific type of substitution among mutated BCRs, demonstrating bias for G to A and C to T transitions.