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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Immunol. 2019 Oct 21;203(11):2817–2826. doi: 10.4049/jimmunol.1900404

Figure 5: Adoptive transfer model confirms memory phenotype of CD11c+ B cells.

Figure 5:

(A, B) VLP Memory model: Anti-VLP IgM (A) and IgG (B) titers after immunization of WT mice with Qβ-VLP, followed by empty Qβ-VLP (ssRNA-free) booster at 100 days. As predicted, secondary immunization with empty VLP induced an enhanced anti-VLP IgG response, but no significant increase in IgM titers. Empty Qβ-VLP alone (without primary immunization) resulted in minimal anti-VLP IgM or IgG. (C) Diagram of experimental strategy showing timing of primary Qβ-VLP immunization, adoptive transfer of CD11c+ B cells into naïve recipients, and secondary immunization with empty Qβ-VLP. (D) ELISA dilution curves showing rapid appearance of anti-VLP IgG titers between days 4 and 6 after empty Qβ-VLP immunization, in animals adoptively transferred with VLP-primed CD11c+ B cells (lower panels). Control animals without CD11c+ transfer developed minimal anti-VLP IgG Ab (upper panels). (E) Anti-VLP IgM (upper) and IgG (lower) titers on indicated days after empty Qβ-VLP immunization. Error bars indicate S.E.M. *, P<0.05; **, P<0.01; by Kruskal-Wallis one-way ANOVA test.