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. 2019 Oct 31;21:720–735. doi: 10.1016/j.isci.2019.10.064

Figure 3.

Figure 3

Increase in Cacna1e and Kcnmb2 with vitE Deficiency in TH+ DRG

(A–D) Green: Th, Blue: DAPI nuclei, Red: Cacna1e [(A) mRNA, (B) protein] and Kcnmb2 [(C) mRNA, (D) protein]. White box inset magnified in the last column. Fluorescent immunohistochemistry from 4-month WT, DEF, and SUPP mice, n = 1–2 per group. Scale bars represent 10 μm (TH, Cacna1e, Kcnbm2, and merge) and 3 μm (enlarged).

(E) Quantification of mRNA using RNAscope for Cacna1e and Kcnmb2. Mean ± SD, N = 8 counts per experimental group, one-way ANOVA, or Kruskal-Wallis. Scale bars represent 10 μm (TH, Cacna1e, Kcnbm2, and merge) and 3 μm (enlarged).

(F) Von Frey assay, demonstrating a significant increase in sensitivity (i.e. lower Dixon's score) in the SUPP vs. DEF mice. Mean ± SD, N = 8–22 per group, one-way ANOVA, ****p< 0.0001, **p< 0.01, *p< 0.05.