Fig. 3.

Suppression of mineral resorption byL.reuteri. RAW264.7 cells were plated for on Osteoassay plates for 1 day and then stimulated concurrently with RANKL (100 ng/ml) and either the vehicle control (MEM-α) or L. reuteri CCS. Fresh medium was replenished every 2 days. The analysis was performed using the Image J software package to measure densitometry. (A) L. reuteri CCS significantly decreased the amount of absorption from calcium and phosphate coated plates. (B) The microscopy images also support that less absorption had taken place in the presence of L. reuteri CCS. Three biological replicates are depicted with associated standard error of mean, *p < 0.05 compared to untreated and MEM-α (vehicle control) conditions as determined by one-way ANOVA. Scale bars signify 100 μm increments. n = 3.