Fig. 8.

Effect ofL.reuteri on RANKL-induced NF-κB/p65 phosphorylation. RAW264.7 cells were concurrently treated with RANKL (100 ng/ml) and MEM-α or L. reuteri CCS for 60 min. Total intracellular contents were prepared and an equal amount of protein was analyzed. Western blot analysis using antibodies to the phosphorylated NF-κB/p65 subunit was performed. Band intensity was measured by densitometry using the ImageJ software package. Results were normalized to levels of total p65. Four biological replicates are depicted with associated standard error of mean, *p < 0.05 compared to untreated and MEM-α (vehicle control) conditions as determined by one-way ANOVA.