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Selection of subjects in this study. A total of 12,250 subjects had a free T4 level within the manufacturer’s normal reference range and were used in the current study as subjects with euthyroxinemia. Some subjects (1601 men and 1203 women) underwent tests for TPO-Ab. A total of 5528 of 12,250 subjects with euthyroxinemia, including 3676 men and 1852 women, revisited our hospital for annual health checkups and were followed up for 1 year
