Fig. 4.

(A) Western blots demonstrated that purified stevioside enhanced the activity of 5-FU by inducing caspase cleavage in MDA-MB-231 cells and also it increases bax/Bcl-2 ratio. Cell lysates were prepared after treating MDA-MB-231 cells with combination and alone treatment after 48 h and examined by anti-caspase antibodies. (B) DNA fragmentation was visualized on agarose gel. All experiments were done in triplet to confirm the reproducibility.