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. Author manuscript; available in PMC: 2019 Nov 20.
Published in final edited form as: Cell Stem Cell. 2008 Sep 11;3(3):279–288. doi: 10.1016/j.stem.2008.07.025

Figure 3. Quantification of the Distance of SVZ Cell Types to Blood Vessels.

Figure 3

(A) Histogram depicting the distribution of distances to blood vessels for each SVZ cell type. (Green) Transit-amplifying C cells. (Blue) Label-retaining astrocytes. (Gray) Nonlabel-retaining astrocytes. (Red) Neuroblasts.

(B) Histogram depicting the first 15 μm of the distributions shown in (A) for each SVZ cell type. 49.0% of EGFR immunoreactive cells and 27.8% of label-retaining astrocytes are found within 5 μm of the vasculature, whereas only 13.9% of neuroblasts are. Pairwise comparisons of the distribution of distance measurements for all cell types revealed statistically significant differences (p < 0.05) between all cell types, except for neuroblasts and nonlabel-retaining astrocytes, which did not differ significantly (p = 0.085, Wilcoxin rank sum test).

(C) Histogram of the average distance (in μm) between individual cells and the nearest blood vessel for neuroblasts (18.3 μm), GFP+ nonlabel-retaining astrocytes (16.6 μm), GFP+ BrdU label-retaining astrocytes (11.2 μm), and C cells (8.8 μm). Error bars represent 2 × SEM.

(D) Z stack projection of whole-mount SVZ immunostained with antibodies against CD31 (red) and the neuroblast marker doublecortin (DCX, blue). In some instances, neuroblasts migrate short distances parallel to blood vessels (arrows). However, while they often cross over blood vessels (arrowheads), the majority of neuroblasts are less closely associated with the vasculature. Scale bar, 100 μm.