Fig. 2.

Exposure to ionizing radiation increases the level of nuclear AR expression. AR levels in the nucleus and cytoplasm of murine PTEN^−/− (A) and human (LNCaP) cells (B) were determined by immunoblot analysis 1 hr following exposure to 0, 2, or 6 Gy. (C) TMPRSS2 expression in LNCaP cells was examined by PCR analysis 1 hr following irradiation. (D) Expression levels of AR RNA and protein in LNCaP cells 1 hr post exposure to 0 or 6 Gy radiation were determined by PCR and immunoblot analyses using whole cell lysate (WCL), respectively. (E) Parental LNCaP cells were treated with vehicle (Veh) or MGI32 prior to exposure to 0 or 6 Gy radiation and AR levels in the nucleus and cytoplasm I hr post radiation were determined by immunoblot analysis. Histone HI and total Akt proteins were used as loading controls for nuclear and cytoplasmic fractions, respectively.