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. 2019 Oct 29;24(21):3903. doi: 10.3390/molecules24213903

Figure 1.

Figure 1

Effect of 1,25-dihydroxyvitamin D (1,25(OH)2D) on cell viability, oxidative stress, lipid peroxidation, and cellular damage in tertiary butyl-hydrogen peroxide-treated C2C12 muscle cells. On day 6 after differentiation induction, C2C12 muscle cells were incubated with tBHP (0.1 mM, 24 h) and treated with 1,25(OH)2D for additional 24 h in a dose-dependent manner (0, 1, 10, or 100 nM). The dissolved blue formazan (A), reactive oxygen species (ROS) (B), lactate dehydrogenase (LDH) levels (C), and lipid peroxidation (MDA) (D) were measured and expressed as the fold change compared to vehicle control. Data are expressed as mean ± SEM. Experiments represent at least two or three independent experiments (n = 6–14 per group). * p < 0.05, ** p < 0.01 compared to vehicle control. # p < 0.05; ## p < 0.01 compared to tBHP control.