Figure 3.
Decolorization percentage of RBBR under three mediators (a) and four metal ions (b). The final laccase activity of the reaction system was 1000 U L−1. Decolorization percentage was determined by measuring changes in absorbance at 592 nm. Reaction condition: pH 4.5, dye concentration 200 ppm, temperature 30 °C, and reaction time 30 min. The error bars represent the standard deviation of triplicate samples. Different letters above bars indicate statistically significant differences between groups according to one-way ANOVA (n = 3, p < 0.05; refer in-text for test specifics and test statistics).