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AAV2 bound to its high-affinity receptor AAVR enhanced viral transduction. (A) AAVR (green) concentrated in the trans-Golgi regions (white) and colocalized with AAV2 (red) in HeLa cells. (B) Western blot of the AAVR in HeLa cells transfected with an AAVR overexpression plasmid. (C) The transduction of AAV2–luciferase (m.o.i = 2000 and 10,000 v.g./cell) was measured at 24 h post-infection in both control and AAVR-overexpressed HeLa cells (** p < 0.01, n = 4).
