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AAVR-SBP-His proteins used for viral overlay assays. (A) Assay workflow. AAV particles were loaded to nitrocellulose (NC) filter, probed with AAVR-SBP-His protein or A20 antibody overnight, and then incubated with HRP conjugated SAV or IgG-HRP. (B) AAV2 of 1 × 109 v.g. subjected to the detection procedure as described in (A). PBST buffer was used as a negative control.
