Table 1.

Optimization of assay conditions for VIRELISA.

		AAV2 (450 nm)	Buffer (450 nm)	P/N
AAVR-SBP-His (μg/mL)	50	0.662 ± 0.003	0.192	3.4
	100	0.861 ± 0.003	0.372	2.3
SAV-HRP dilution	1/1000	0.872 ± 0.006	0.598	1.5
	1/2000	0.798 ± 0.006	0.231	3.5
	1/5000	0.662 ± 0.001	0.171	3.9
ABST incubating time (min)	10	0.320 ± 0.005	0.12	2.7
	15	0.663 ± 0.007	0.19	3.5
	20	0.819 ± 0.002	0.25	3.3
	30	1.326 ± 0.060	0.42	3.1