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Kinetic parameters for nucleotide incorporation opposite Fm7dG and dG by polymerase η (polη).
| Template:dNTP |
Km (μM) |
kcat (10−3 s−1) |
kcat/Km (10−3 s−1μM−1) |
f a | Replication Fidelity |
|---|---|---|---|---|---|
| dG:dCTP | 2.66 ± 0.29 | 120.6 ±6.1 | 45.6 | 1 | |
| dG:dTTP | 159.3 ± 2.7 | 74.8 ± 0.9 | 0.47 | 0.01 | 97 |
| Fm7dG:dCTP | 4.27 ± 0.42 | 56.4 ± 2.7 | 13.2 | 1 | |
| Fm7dG:dTTP | 52.5 ± 1.7 | 49.3 ± 0.05 | 0.94 | 0.07 | 14.3 |
a Relative efficiency: (kcat/Km)[dTTP:dG]/(kcat/Km)[dCTP:dG] or (kcat/Km)[dTTP:Fm7dG]/(kcat/Km)[dCTP:Fm7dG].
