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β-tubulin immunofluorescence analysis in AGS cell line treated with K858 for 24 h. AGS cells were treated for 24 h with 1 and 3 μM of K858. Tubulin cytoskeleton was visualized following fixation by immunostaining, DNA was stained with 4’,6-diamidino-2-phenylindole (DAPI). After 24 h of K858 treatment the majority of AGS cells arrested in mitosis resulting in formation of monopolar spindle (yellow arrow).
