Fig. 5.

MiR-7-5p suppresses tumor growth and metastasis in vivo. a – miR-7-5p suppressed tumor growth, as measured by tumor volume. A549 cells (1 × 10⁷ cells per mouse) were subcutaneously injected into 5- to 6-week old mice. When the tumor had grown to about 100 mm³, the mice were injected intravenously with NC mimic or miR-7-5p mimic every two days for 18 days. b – MiR-7-5p had minimal effect on the body weight of the mice. c – MiR-7-5p inhibited the tumor growth, as measured by tumor weight. d – MiR-7-5p decreased the expression of EMT markers in A549 xenografts. At the end of the experiment, the mice were killed, and the tumors were removed and used for the qPCR assay to determine the mRNA level of N-cadherin, vimentin and E-cadherin. Quantitative data are presented as the means ± SEM. ***p < 0.001 compared with the NC mimic group