Fig. 4.

Increased effector function of CD8+ and CD4+ T cells in tumors of tritherapy-treated mice. a, Representative flow plots and b, graphical representation of intracellular GzA and surface CD107a expression on CD8+ T cells in tumors harvested 7 days after p.t. DR-BMC injections. Pooled data from 3 independent experiments is shown for GzA + CD8+ T cells, and from 2 independent experiments for CD107a + and GzA + CD107a + CD8+ T cells. One-Way ANOVA. c, 10 days after DR-BMC p.t. injections, Line-1 tumors were harvested 4.5 h after BFA i.v. injections and intracellular cytokine staining on CD8+ T-cells was performed. Data shown here is representative of 2 independent experiments. d, Same as c but representative flow plots of tumor-infiltrating CD4+ T cells. e, Same as c-d but cytokine production by tumor CD4+ T cells is shown. Data shown here is representative of 2 independent experiments. One-Way ANOVA