Fig. 2.

RNAi of PAX8 brings to an increased sensitivity to anoikis of ovarian cancer cells and reduced expression of EMT markers. a Following RNAi of PAX8, the survival percentage of anchorage-independent KURAMOCHI cells was compared with that of cells under adherent condition at 24 h and 48 h. Values are mean ± SD of three independent experiments normalized with respect to the cells transfected with the scramble siRNA. p-value was calculated by t-test p ≤ 0.1. b Western blots to evaluate the protein levels of Bcl-2 and Bax after PAX8 depletion. c The expression levels of a panel of mesenchymal markers were measured by qRT-PCR in KURAMOCHI cells 48 h after RNAi of PAX8. The values are mean ± SD of three independent experiments in duplicate, normalized by the expression of ABL and expressed as fold change with respect to the siCTR cells, whose value was set at 1.0. p-value was calculated by t-test 0.001 ≤ p ≤ 0.1