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. 2019 Nov 20;8:181. doi: 10.1186/s13756-019-0637-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The specificity of each primer pair investigated by qRT-PCR for this study. End amplified products of qPCR were visualised using agarose gel electrophoresis. All genes were detected at the expected bp size and single band was observed for each primer pair indicating primer specificity. M, Molecular Weight marker, GeneRuler 50 bp DNA Ladder (Thermofisher, UK). Products and ladder ran on 4% Agarose gel.